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| Cat.No.: | Kit-0535 |
| Product Overview: | Maleimide Assay Kit (Rapid) is a quantitative assay for the quantification of maleimide groups. |
| Description: | A variety of crosslinking reagents with a maleimide group are widely used for crosslinking proteins to proteins, or proteins to other biomolecules. A key challenge in maleimide crosslinking technology has been the development of reliable and reproducible methods for quantitation the amount of maleimide linked to a single protein or antibody. Maleimides can be directly assayed spectrophotometrically at 302 nm. However, the small extinction coefficient of 620 M-1cm-1 renders this assay insensitive, and the assay is further complicated by the protein absorbance at the same wavelength. There are a few methods available for quantitating the number of maleimide groups by indirect ways. And in many cases, the maleimide ratio is only estimated by experience. |
| Applications: | Maleimide Assay Kit (Rapid) provides an accurate method to quantify maleimide group by our maleimide sensor Maleimide Blue with the maximum absorbance at ~780nm. |
| Usage: | For research use only (RUO) |